Embryo vitrification using a novel semi-automated closed system yields in vitro outcomes equivalent to the manual Cryotop method
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چکیده
منابع مشابه
Embryo vitrification using a novel semi-automated closed system yields in vitro outcomes equivalent to the manual Cryotop method.
STUDY QUESTION Can the equilibration steps prior to embryo vitrification be automated? SUMMARY ANSWER We have developed the 'Gavi' system which automatically performs equilibration steps before closed system vitrification on up to four embryos at a time and gives in vitro outcomes equivalent to the manual Cryotop method. WHAT IS KNOWN ALREADY Embryo cryopreservation is an essential componen...
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OBJECTIVE To compare the mouse oocyte vitrification outcomes of the CryoLogic vitrification method (CVM) and the conventional open method using a Cryotop. Two CVM methods (original CVM and modified CVM) were tested. METHODS Mature oocytes obtained from female BDF-1 mice were vitrified by two-step exposure to equilibrium and vitrification solutions. Three vitrification protocols were tested on...
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متن کاملThe effect of vitrification on mouse oocyte apoptosis by cryotop method.
BACKGROUND Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. METHOD A total of 200 germinal vesicle (...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2014
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/deu214